DNA fingerprinting: improved DNA extraction from small blood samples
نویسندگان
چکیده
منابع مشابه
DNA fingerprinting: improved DNA extraction from small blood samples.
Since DNA fingerprinting has become widely used ' 2 3 kb in genetic analysis of different species the original method (1) has undergone modifications, e.g. in DNA extraction, Southern transfer and labeling of the DNA probe. While applying this technique for pedigree monitoring of mouse inbred strains, it was important to isolate DNA from small blood samples in order to leave the animals alive. ...
متن کاملGenomic Dna Extraction from Dried Blood Spots
We have developed an efficient and simple method for extracting and purifying genomic DNA from dried blood stored on filter paper. The quality of the genomic DNA extracted is tested by PCR amplification of a 255-bp fragment of the PAX8 gene sequence and the PCR products are determined for further genetic studies by single strand conformation polymorphism (SSCP) analysis. Larger DNA sequences of...
متن کاملexamination and comparison of various methods of dna extraction from samples of blood, bone marrow slides and peripheral blood
introduction: dna extraction methods are very important for genetics research and diagnostic tests. in these methods are also important for detecting genetic diseases or in cancers for investigating genetic changes during cancer progression or treatment. therefore, selection of the best method for dna extraction from different samples such as bone marrow (bm) or peripheral blood (pb) and their ...
متن کاملImproved extraction of PCR-quality community DNA from digesta and fecal samples.
Several DNA extraction methods have been reported for use with digesta or fecal samples, but problems are often encountered in terms of relatively low DNA yields and/or recovering DNA free of inhibitory substances. Here we report a modified method to extract PCR-quality microbial community DNA from these types of samples, which employs bead beating in the presence of high concentrations of sodi...
متن کاملAn Improved Method for High Quality Metagenomics DNA Extraction from Human and Environmental Samples
To explore the natural microbial community of any ecosystems by high-resolution molecular approaches including next generation sequencing, it is extremely important to develop a sensitive and reproducible DNA extraction method that facilitate isolation of microbial DNA of sufficient purity and quantity from culturable and uncultured microbial species living in that environment. Proper lysis of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1988
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/16.15.7738